This study investigated bovine TRL4 gene c.9421C>T, c.2021C>T and c.-10C>T polymorphisms and their relationship with somatic cell count and indicators of milk composition. Blood samples were collected from 152 Lithuanian Holstein dairy cows. The method of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) was used to genotype TLR4 gene polymorphisms. The data concerning somatic cell count and milk composition indicators (fat, protein, lactose) were analysed. The influence of genes and statistical significance of differences between different genotypes was evaluated by the one-factor dispersion analysis (ANOVA). Results showed that c.9421C>T was significantly associated with somatic cell count in milk. Also a significant association between the genotypes of c.9421C>T and somatic cell count was found (P<0.05). Cattle with the TT genotype showed the lowest somatic cell count. TLR4 gene polymorphisms c.2021C>T and c.-10C>T have no significant effect on mastitis resistance and milk composition. Analysis of the combined genotypes TC/CC of c.2021C>T and c.9421C>T allowed us to determine the possible association of SNPs with the somatic cell count and lactose content. The study showed a significant association between the somatic cell count (SCC) and TLR4 polymorphism c.9421C>T in Lithuanian Holstein cows. SCS of cows with a TT genotype was significantly lower and indicated the association of TT genotype with resistance to mastitis in c.9421C>T and allele T might be the beneficial allele for mastitis resistance.; U okviru ove studije ispitivani su polimorfizmi c.9421C>T, c.2021C>T i c.-10C>T kravljeg gena TLR4 te njihova povezanost s brojem somatskih stanica i sastavom mlijeka. Uzorci krvi izuzeti su od ukupno 152 krave holstein pasmine u Litvi. Za tipizaciju polimorfizma gena TLR4 korištene su metode polimeraza lančane reakcije (PCR) i polimorfizam dužine restrikcijskih fragmenata (RFLP). Također su određivani broj somatskih stanica i odabrani parametri kemijskog sastava mlijeka (mast, protein, laktoza). Za određivanje utjecaja gena i statističkog značaja razlika među ispitivanim genotipovima korištena je jednosmjerna analiza varijance (ANOVA). Rezultati su pokazali da postoji statistička značajna povezanost između genotipa c.9421C>T i broja somatskih stanica (P<0,05). Jedinke genotipa TT imale su najniži broj somatskih stanica. Polimorfizmi c.2021C>T i c.-10C>T gena TLR4 nisu pokazali značajan utjecaj na otpornost prema mastitisu niti na sastav mlijeka. Analiza kombiniranih genotipova TC/CC polimorfizma c.2021C>T i c.9421C>T omogućili su utvrđivanje povezanosti SNP-ova s brojem somatskih stanica i s udjelom laktoze. Ovim istraživanjem je utvrđena značajna povezanost između broja somatskih stanica i polimorfizma c.9421C>T gena TLR4 u krava pasmine holstein u Litvi. Broj somatskih stanica u krava genotipa TT bio je značajno niži i ukazivao je na povezanost ovog genotipa s otpornošću na mastitis u c.9421C>T. Stoga se alel T može smatrati poželjnim za otpornost prema mastitisu.