Cilj ovog rada bio je istražiti korisnost upotrebe imunohistokemijske metode (IHK) zajedno s histopatološkom pretragom u dijagnosticiranju i razlikovanju alimentarnog limfoma (AL) od upalne bolesti crijeva (UBC). U istraživanju su korišteni arhivski histopatološki nalazi i preparati tkiva, parafinski blokovi i stakalca. Prema pripadnosti pasmina podijelili u tri skupine malu, srednju ili veliku skupinu pasa. Cilj istraživanja bio je utvrditi povezanost pojedinih patoloških promjena ovisno o skupini pasa, dobi i spolu. Obavljenim istraživanjem zabilježili smo da je srednja vrijednost dobi u skupini malih pasmina pasa iznosila 7,58±3,59 godina, u skupini srednjih pasmina 7,45±3,04 godina te u skupini velikih pasmina 6,58±3,47 godina. Prosječna dob pasa oboljelih od alimentarnog limfoma iznosila je 8,8 godina, za razliku od upalne bolesti crijeva gdje je iznosila 7,0 godina. Unutar sve tri skupine pasa zabilježen je veći broj jedinki muškog spola. Najveći udio biopsiranih uzoraka (70,73 %) analiziran je iz tkiva tankog crijeva; po 8 uzoraka (27,59 %) iz male skupine pasa, 11 uzoraka iz srednje skupine (37,93 %) te po 10 uzoraka iz velike skupine pasa (34,48 %). Udio biopsiranog tkiva želudca bio je nešto niži (24,39 %) dok je tkivo debelog crijeva uzorkovano u samo dva psa (4,88 %). Histopatološkom analizom utvrđeno je 16 slučajeva upalne bolesti crijeva (45,71 %), 7 slučajeva limfoma (20,00 %), 9 slučajeva suspektne upale (25,71 %) te 3 slučaja suspektne upale/limfoma (8,58 %). Nakon provedene IHK metode ustanovljeno je da je u 6 slučajeva dijagnoza donesena histopatološkom evaluacijom potvrđena, u 5 slučajeva je opovrgnuta dok je u 5 slučajeva IHK metoda bila korisna kako bi razlučili AL od UBC. Utvrđene su značajne razlike (P<0,05) između dijagnoze upalne bolesti crijeva te limfoma ustanovljene patohistološkom pretragom te imunohistokemijskom metodom.; The aim of this study was to evaluate the use of immunohistochemistry (IHC) with the histopathological method to diagnose and differentiate alimentary lymphoma (AL) from inflammatory bowel disease (IBD). Archival histopathological findings and tissue samples, paraffin blocks and slides were used. This study included 35 dogs divided in three groups regarding breed size - small, medium or large - aiming to determine the correlation between certain pathohistological changes depending on breed, age and gender. The average age of dogs in the small breeds group was 7.58±3.59 years, in the medium breeds group was 7.45±3.04 years and in the large breeds group was 6.58±3.47 years. The average age of dogs suffering from AL was 8.8 years, unlike dogs suffering from IBD, with the average age of 7 years. A larger number of males was represented within all three groups. The highest share of biopsy samples (53.65%) involved the analysis of small intestine tissue, with eight samples (33.33%) in all three groups. The portion of the biopsied stomach tissue was somewhat lower (21.95%), while in two breeds (4.87%) colon tissue was sampled. Histopathological analysis determined 16 cases of IBD, seven cases of lymphoma (20.00%), nine cases of suspected inflammation (25.71%) and three cases of suspected inflammation/lymphoma (8.58%). After IHC, the diagnosis determined by histopathological evaluation was confirmed in six cases, rejected in five cases, while in five cases it was useful in distinguishing AL from IBD. Significant differences (P<0.05) were found between the diagnosis of IBD and AL ascertained by pathohistological analysis and the IHC method. T cellular lymphoma was also found to be more common in the small intestine, while B cellular lymphoma was more prevalent in the stomach.