High performance liquid chromatographic analysis of rutin in Allium species from Bosnia and Herzegovina / Mirsada Salihović, Emin Sofić.
Sažetak

Rutin is quercetin heteroside widely present in plants that exhibit many health-beneficial effects, such as strengthening the capillary walls, reducing the harmful effects of LDL cholesterol, and reducing the risk of cardiovascular diseases. Rutin was determined from three Allium species (A. cepa, A. sativum, and A. ursinum) collected in Bosnia and Herzegovina by the HPLC method associated with electrochemical detection. The analysis was performed from methanol extracts of bulb and leaf of garlic, bulb and leaf of onion, and leaves of wood garlic. Rutin was present in all of the examined samples. The highest rutin content was found in garlic leaves (0.78 ± 0.09 mg g–1), and the lowest in onion bulbs (0.04 ± 0.10 mg g–1). The contents of rutin were higher in leaf samples, suggesting that leaves of onion and garlic are recommended as a better natural source of this glycoside.; Rutin je heterozid kvarcetin široko rasprostranjen u biljkama i pokazuje brojne pozitivne učinke na zdravlje, poput jačanja stijenki kapilara, smanjenja štetnih učinaka LDL kolesterola i smanjenja rizika od bolesti krvožilnog sustava. Udio rutina određen je u tri vrste roda Allium (A. cepa, A. sativum i A. ursinum) koje rastu u Bosni i Hercegovini primjenom tekućinske kromatografije visoke učinkovitosti s elektrokemijskom detekcijom (HPLC-ECD). Analiza je provedena u metanolnim ekstraktima lukovice i lista češnjaka, lukovice i lista crvenog luka, lista medvjeđeg luka. Rutin je određen u svim analiziranim uzorcima vrste Allium. Najveći sadržaj rutina određen je u listovima češnjaka (0.78 ± 0.09 mg g–1), a najmanji u crvenom luku (0.04 ± 0.10 mg g–1). Udjeli rutina bili su veći u uzorcima lišća, što sugerira da se listovi luka i češnjaka preporučuju kao bolji prirodni izvor ovog glikozida.