Histidine monolayer was adsorbed on the surface of the gold electrode and further tailored with Cu2+ cation with the aim to investigate adsorption of histidine (His) and human serum albumin (HSA). Formation of the layer on the surface of the gold electrode was confirmed with quartz crystal microbalance. Binding of the His and HSA onto the modified electrode was successfully done for a wide range of tested concentrations. Electrode response was linearly proportional to the concentration of His and HSA with the correlation coefficients R2 = 0.9895 and R2 = 0.9952 respectively.; Histidinski monosloj adsorbiran je na zlatnu elektrodu i dalje modificiran kationom Cu2+. Cilj je bio istražiti vezanje histidina (His) i humanog albumina (HSA) na tako pripremljenu površinu. Formiranje sloja na površini zlatne elektrode potvrđeno je kvarcnom mikrovagom. Vezanje histidina i humanog albumina na modificiranu elektrodu uspješno je izvršeno za širok raspon ispitivanih koncentracija. Dobiven je odziv linearno proporcionalan koncentraciji histidina i humanog albumina s koeficijentom korelacije R2 = 0,9895 odnosno R2 = 0,9952.